Cloning and In Vitro-Transcription of Chymosin Gene in E. coli
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منابع مشابه
Cloning and In Vitro-Transcription of Chymosin Gene in E. coli
Chymosin, commonly known as rennin, is the main milk-coagulating enzyme available in rennet. RNA was extracted from the abomasum of a suckling calf water buffalo and was subjected to RT-PCR using degenerate primers to amplify 850bp of the chymosin gene. The sequence was aligned with 19 different mammals' chymosin genes. The sequence revealed that there is a similarity to them ranging from 64% t...
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Human growth hormone (hGH) genomic sequence containing 5 exons and 4 introns was cloned in pcDNA-3 and the constructed plasmid was subsequently used for transfection ofNlli-3T3 cell line using lipofection technique. Expression of hGH in stably transfected cells was assayed using ELISA. Total RNA was extracted from transfected cells and hGH cDNA was amplified by RT-PCR using specific primers...
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ژورنال
عنوان ژورنال: The Open Nutraceuticals Journal
سال: 2010
ISSN: 1876-3960
DOI: 10.2174/18763960010030100063